Madden NFL 2000 is a football video game. This was the second of the Madden NFL games to not solely feature John Madden on the cover in North America. The only other one was Madden NFL '95. Most versions of the game cover featured Madden prominently in the foreground, and a recognizable Barry Sanders in a background action graphic. The European PAL edition features only Dorsey Levens on the cover.

The video at the introduction on the CD-ROM versions of the game is an unnamed track by rapper Ludacris.

It was the first Madden game to be released for the Game Boy Color. It was also the first Madden game to be released on both PC and Mac.

The Green Bay Packers had the best team overall in the game with a score of 94. The worst team in the game was the Cleveland Browns with a score of 68. The best offense in the game belongs to the Denver Broncos with a score of 97. The best defense in the game belongs to the Miami Dolphins with a score of 96. The best special teams in the game belongs to both the Buffalo Bills and Atlanta Falcons with a score of 97.

A limited number of players or their names can now be either created or edited as modified rosters.

Franchise mode had improved somewhat and multiple owners can now participate in the same season. Unlike the previous version, Madden NFL 2000 allows the user to choose if he wants the computer to make his off-season decisions, such as re-sign/release players, draft future stars, etc. For the second straight year, created players can not be added to their respective teams, as well as to the list of free agents.